Do watch the rest of my semen analysis videos by clicking on below links. Do click on Hashtags above video title for more of my biology experiment videos. Semen analysis Complete Video: Semen Analysis 1-6 Liquefaction time, Volume, Colour, pH, Odour, Viscosity: Semen analysis Sperm Motility Test: Semen analysis Sperm Viability Test: Semen Analysis Sperm Morphology: Semen analysis Sperm Count: Semen analysis Fructose content: Eosin and Nigrosin Stain Preparation (for Viability Test): #MedicalLab #ReproductiveBioLab #ThomasTKTungnung #CytologyLab #AnimalPhysioLab Fructose is produced in the seminal vescicles and constitutes about 99% of the reducing sugars present in semen. It is the main source of energy for the sperm cells. Semen samples normally contain fructose and can be detected by qualitative analysis using Seliwanoff’s reagent. Quantitative colorimetric estimation can also be performed to find out the exact amount of fructose present in the sample. Fructose test is an optional parameter in routine semen anlysis, and is performed especially when the semen sample contains no sperm cells, a condition known as Azoospermia, or when ejaculate volume is below normal. Qualitative Fructose test is performed using Seliwanoff’s reagent. The test is based on the principle that when fructose is heated with resorcinol in an acidic medium (both components of Seliwanoff’s reagent), fructose is converted to hydroxymethyl furfural, which then condenses with resorcinol to form a red precipitate. To perform qualitative test for fructose in semen sample , take 5ml of Seliwanoff’s reagent in a test tube. Add 0.5ml of the semen sample to the test tube and boil the mixture for a minute or so. The formation of a red colour in the tube is considered as positive test for fructose. No change in colour indicates an absence of fructose in the sample. To know the exact amount of fructose present in the sample, Quantitative fructose test may be further performed in a colorimeter using standard fructose and resorcinol reagent. Semen Fructose content of 200-400mg/dl is considered normal. Low or No fructose levels in semen is indicative of conditions such as – testosterone deficiency, – obstruction, complete absence or developmental defects of seminal vescicles and/or vas deferens